Misha, the nine-year-old aardvark, has been killed in a fire broke out in a shop, and a café on Saturday at the London Zoo.
The zoo, which closed for the rest of the day, declared that four meerkats also died in the incident.
The zoo said: "Our veterinarians closely observe the case of all other animals in the area, and the early signs suggest they are not damaged.”
The fire broke out at 06:00 GMT in the Animal Adventures section and spread to a nearby shop.
Ten fire engines, 72 firefighters and an anti-fire officer were called, and the London Fire Department said the blaze was controlled within three hours.
The zoo said in a statement that the resident staff immediately began moving the animals to safe places, adding that a number of employees had been treated from smoke inhalation and trauma.
"The fire has mainly affected the cafe and the shop, and part of a nearby area used to pamper animals have also been damaged," said London Fire Brigade (LFB) station manager Clive Robinson.
The cause of the fire is being investigated. The London Zoo said on Twitter it had closed its doors on Saturday.
It reopened on Sunday.
The Regent Park Zoo was opened to the public in 1846. The 36-acre facility has about 2,166 animals and birds according to the last inventory conducted in January.