Serena Williams withdrew from the French Open on Wednesday ahead of her second round match with an Achilles injury, as the American's bid for a 24th Grand Slam title suffered another blow.
The 39-year-old Williams, a three-time winner at Roland Garros, had arrived in Paris carrying an Achilles injury that prompted her to skip the Rome tune-up event.
"The Achilles didn't have enough time to heal after the US Open," said Williams.
"I was struggling to walk and that is a tell-tale sign that I should try to recover."
The injury likely means she will miss the rest of 2020 leaving the Australian Open in 2021 as her next chance to equal Margaret Court's all-time majors record.
"I need four to six weeks of sitting and doing nothing," she said. "It's more than likely that I won't play another tournament this year."
Her decision to pull out handed Bulgaria's Tsvetana Pironkova a walkover and left Williams searching for a first major since the last of her 23 titles at Melbourne in 2017.