Israelis were prevented from entering Jordanian border enclaves on Sunday after a deal allowing farmers to work land there expired, 25 years after a peace treaty.
Since the two countries signed the deal in 1994, Jordan has allowed Israeli farmers access to the territories of Ghumar in the north and Baqura in the south under a renewable 25-year arrangement.
In October last year however, Jordan's King Abdullah said his country had notified Israel that it wants them back.
An AFP journalist in northern Israel on Sunday said the yellow gate to a bridge over the river dividing the two countries, long the portal for Israeli farmers to access Baqura, was locked.
Baqura, known in Hebrew as Naharayim, lines on a spit of land where the Jordan and Yarmuk rivers meet.
Ghumar, deep in the Negev desert south of the Dead Sea, is known in Hebrew as Tzofar.
The lands have been privately owned by Israeli entities for decades, but the 1994 deal saw the kingdom retain sovereignty there.
Israel's relations with Amman been increasingly strained.
Opinion polls have repeatedly found that the peace treaty with Israel is overwhelmingly opposed by Jordanians, more than half of whom are of Palestinian origin.
In 2017, an Israeli embassy security guard in Amman killed two Jordanians.
Three years earlier, an Israeli soldier at a border crossing killed a Jordanian judge he deemed a threat.
Just last month, Amman recalled its ambassador from Israel over the prolonged detention without trial in Israel of two Jordanians. The ambassador returned after the two were released.